HPLC Column, NP Amino, 3um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent NP Amino™ is a normal-phase and HILIC stationary phase designed for the retention and separation of highly polar compounds. Manufactured using a three-carbon propyl ligand with a terminal amino functional group bonded to high-purity silica, this stationary phase provides selectivity that is particularly useful for carbohydrates, organic acids, and other polar analytes. As a USP L8 equivalent phase, it is widely used as an alternative in established USP methods and for new method development involving Normal Phase and HILIC chromatography.
68340-25P-4 is an HPLC column packed with 3 µm NP Amino™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the NP Amino product family, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for the analysis of sugars, carbohydrates, compounds containing carboxylic acid groups, and other polar molecules. Suitable for both Normal Phase and HILIC chromatography, the amino functionality provides retention characteristics that make it valuable for analytes that are poorly retained on conventional reversed-phase columns. As an unmodified silica bonded amino phase, this column is also suitable for HILIC applications.
Key Benefits
NP Amino™ stationary phase
USP L8 equivalent chemistry
Amino bonded phase
High-purity silica support
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
3 µm particle size
Greater efficiency than 5 µm columns
Maximum chromatographic resolution
Typical Applications
USP L8 methods
Sugar analysis
Carbohydrate analysis
Organic acid analysis
Polar compound analysis
HILIC methods
Normal Phase chromatography
Food and beverage analysis
Pharmaceutical analysis
Method development
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™
USP Classification: L8
Chromatography Modes: Normal Phase and HILIC
Bonded Phase: Amino
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 100 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for highly polar compounds
Commonly used for sugars and compounds containing carboxylic acid groups
Suitable for both Normal Phase and HILIC chromatography
As an unmodified silica bonded amino phase, this column is suitable for HILIC applications
The 250 mm length provides maximum resolution and peak capacity for demanding separations
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
Packed and shipped in Normal Phase solvents
Provides an excellent alternative for USP L8 methods
Particularly useful for complex separations requiring maximum chromatographic resolution
This is a Special Order Item